免费通用引物列表

引物名称 序列(5’to3′) 碱基数(mer)
M13F(-77) GATGTGCTGCAAGGCGATTA 20
M13R(-88) TTATGCTTCCGGCTCGTATG 20
M13F(-47) CGCCAGGGTTTTCCCAGTCACGAC 24
M13R(-48) AGCGGATAACAATTTCACACAGGA 24
T7 TAATACGACTCACTATAGGG 20
T7t TGCTAGTTATTGCTCAGCGG 20
SP6 ATTTAGGTGACACTATAG 18
T3 ATTAACCCTCACTAAAGGGA 20
pGEX-5 GGGCTGGCAAGCCACGTTTGGTG 23
pGEX-3 CCGGGAGCTGCATGTGTCAGAGG 23
pEGFP-N-5 TGGGAGGTCTATATAAGCAGAG 22
pEGFP-N-3 CGTCGCCGTCCAGCTCGACCAG 23
CMV-Forward CGCAAATGGGCGGTAGGCGTG 21
U6-promotor ATGGACTATCATATGCTTACCGTA 24
pEGFP-N-3’ CGTCGCCGTCCAGCTCGACCAG 22
pEGFP-N-5’ TGGGAGGTCTATATAAGCAGAG 22
pEGFP-C-3’ TATGGCTGATTATGATCAGT 20
pEGFP-C-5’ CATGGTCCTGCTGGAGTTCGTG 22
pFastBac-F GGATTATTCATACCGTCCCA 20
pFastBac-R CAAATGTGGTATGGCTGATT 20
3’AD AGATGGTGCACGATGCACAG 20
BGHR TAGAAGGCACAGTCGAGG 18
S-Tag CGAACGCCAGCACATGGACA 20
RVP4 GACGATAGTCATGCCCCGCG 20
pQE30-R GTTCTGAGGTCATTACTGG 19
pQE30-F TGAGCGGATAACAATTTCAC 21
5-AOX1 GACTGGTTCCAATTGACAAGC 21
3-AOX1 GCAAATGGCATTCTGACATCC 21
RSFDuet-1-seqF CATCACCATCATCACCACAGCCAGG 25
pTrcHis-F GAGGTATATATTAATGTATCG 21
pTrcHis-R GATTTAATCTGTATCAGG 18
M13F/M13(-21) TGTAAAACGACGGCCAGT 18
M13R CAGGAAACAGCTATGAC 17
27F AGAGTTTGATCCTGGCTCA 19
1492R GGTTACCTTGTTACGACTT 19
EF3 TCCTCTAAATGACCAAGTTTG 21
EF4 GGAAGGGRTGTATTTATTAG 20
NS1 GTAGTCATATGCTTGTCTC 19
NS8 TCCGCAGGTTCACCTACGGA 20
ITS4 TCCTCCGCTTATTGATATGC 20
ITS5 GGAAGTAAAAGTCGTAACAAGG 22
ITS1 GTAGGTGAACCTGCGG 16
NL1 GCATATCAATAAGCGGAGGAAAAG 24
NL4 GGTCCGTGTTTCAAGACGG 19
pCold-F ACGCCATATCGCCGAAAGG 19
pCold-R GGCAGGGATCTTAGATTCTG 20
v1.5 ?GGACTT TCCAAA ATG TCG 22
XL39 ATTAGGACAAGGCTGGTGGG 20
RVP3 CTAGCAAAATAGGCTGTCCC 20
EF1A-F TCAAGCCTCAGACAGTGGTTC 21
pPIC9K-seqR ATGGCATTCTGACATCCTCTT 21
a-factor TACTATTGCCAGCATTGCTGC 21
3BD TAAGAGTCACTTTAAAATTTGTATC 25
5AD AGGGATGTTTAATACCACTAC 21
??? PCMV-F TCTAAAAGCTGCGGAATTGT 20
??? PCMV-R TCCAAACTCATCAATGTATC 20
pBABE-3 ACCCTAACTGACACACATTCC 21
pBABE-5 CTTTATCCAGCCCTCAC 17
pBAD-Forward ATGCCATAGCATTTTTATCC 20
pBAD-Reverse GATTTAATCTGTATCAGG 18
PBV220F AAGAAGGGCAGCATTCAAAG 20
PBV220R CTGCGTTCTGATTTAATCTG 20
GLP2 CTTTATGTTTTTGGCGTCTTCCA 23
?? PIRES3-EGFP-R AACGCACACCGGCCTTATTC 20
? PIRES-F CTTACTGACATCCACTTTGC 20
PIRES-R CACTGCATTCTAGTTGTGGT 20
? Human-U6 GGGCAGGAAGAGGGCCTA 18
DuetUp2 TTGTACACGGCCGCATAATC 20
DuetDown1 GATTATGCGGCCGTGTACAA 20